Fig. 1.

Schematic of 3T3-L1 preadipocyte differentiation. Fully cultured 3T3-L1 cells were treated with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM dexamethasone, and 10 μg/mL insulin (MDI) for 2 days. The cell culture medium was changed with new 10 μg/mL of insulin-containing Dulbecco’s modified Eagle’s medium every 2 days. To investigate the effects of gongmi tea or gongmi so extract on adipogenesis, differentiated 3T3-L1 cells were treated every 2 days. After 8 days of differentiation, Oil red O staining and Western blot analysis were conducted.