Fig. 3.

Effects of gongmi tea and gongmi so extract on lipid accumulation in adipocytes. The 3T3-L1 cell line was seeded in 6-well plates and grown to full confluence for 2 days. Then, the cells were treated with gongmi tea or gongmi so extract and differentiated into adipocytes via the MDI treatment. The differentiated adipocytes were treated with gongmi tea or gongmi so extract (100 and 300 μg/mL), and SB203580 and garcinia were used as positive controls. (A) Intracellular lipids were stained with Oil red O and then quantified. The administration of gongmi tea and gongmi so extract decreased lipid accumulation. (B) Stained lipid droplets were dissolved in 100% isopropanol, and absorbance was measured at 490 nm. Data are expressed as mean±standard error of the mean. ^###P<0.001 compared with the non-treated group. *P<0.05, **P<0.01, and ***P<0.001 compared with the MDI-treated group. MDI, 3-isobutyl-1-methylxanthine, dexamethasone, and insulin.