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. 2023 Apr 12;222(6):e202208136. doi: 10.1083/jcb.202208136

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© 2023 Jensen et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 2. — Identification of ABI2 as a novel PIM substrate. (a) Experimental strategy for the identification of novel PIM substrates using SILAC. (b) Volcano plot of the phosphopeptides that were significantly increased and decreased, as determined by a one-sample t test in hypoxia after AZD1208 treatment. (c) Graph depicting relative serine phosphorylation of peptides containing Ser183 in the presence and absence of PIM inhibition. Error displayed as 95% CI as determined by one-sided Student’s t test (*P < 0.05). (d) Spectra from mass spectrometry analysis of ABI2 from in vivo kinase assay showing phosphorylation of Ser183 by PIM1. Inset = sequence alignment across species of ABI2 Ser183 (highlighted). (e) ABI2 gene expression in human prostate tumor samples from the National Center for Biotechnology Information GEO database (TNMplot web analyzer). (f) Kaplan–Meier analysis of biochemical recurrence-free survival in PCa patient cohorts based on the expression of ABI2.