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. 2023 Apr 12;222(6):e202208136. doi: 10.1083/jcb.202208136

Figure 6.

Figure 6.

PIM1-ABI2 signaling controls tumor cell invasion in vivo. (a) Representative images of PIM1 expression in non-invasive and invasive tumor seeded on the diaphragm (black dotted line indicates the tumor/diaphragm interface). (b) Representative images of DU145 and ABI2-KOA20 tumors with PIM1 overexpression or PIM447 treatment (white dotted line indicates the tumor/diaphragm interface). (c) Relative tumor burden was quantified based on H&E staining of the total tumor area compared with parental DU145 tumors (n = 12, N = 3). Error = 95% CI as determined by two-sided Student’s t test (*P < 0.05 compared with DU145 vector, *P < 0.05 compared with ABI2-KOA20 vector). (d) Depth of invasion from the tumor/diaphragm interface of individual tumor cells was measured and a Kolmogorov–Smirnov test was run to determine the statistical difference among the distributions (***P < 0.001 as compared with DU145-DMSO treated cells and **P < 0.01 between A20-DSMO and A20-hPIM1 cells). (e) The average depth of invasion was graphed for DU145 (n = 11,951, N = 12), DU145-hPIM1 (n = 12,314, N = 12), DU145-PIM447 (n = 12,547, N = 12), ABI2-KOA20 (n = 1,942, N = 7), and ABI2-KOA20-hPIM1 (n = 5,275, N = 12) tumors. (f) Plot depicting the depth of tumor cell invasion in vivo vs. relative protrusive activity. (g) Gene expression data of PIM1 and ABI2 from benign and metastatic PCa patient samples.