Figure S1.

Loss of PIM inhibits cell motility and spreading. (a) PIM1/2/3 expression in LN4 and DU145 in normoxia and hypoxia. (b) PIM1 expression in LN4 and CRISPR normoxia vs. hypoxia. (c) Boyden Chamber invasion assay of PC3-LN4 cells with or without PIM inhibition under normoxic and hypoxic conditions. The number of invading cells was quantified. Error displayed as SEM (n = 3; *P < 0.05). Scale bar = 200 µm. (d) mRNA expression for PIM1/2/3 in WT and TKO cell lines. (e) Wound healing assay and subsequent quantification of percent wound closure in MEFs with intact or complete loss of PIM kinase. Error is SEM (n = 3; *P < 0.05). Scale bar = 200 µm. (f) WT and TKO cells were placed in dishes and allowed to seed. Cells were imaged every 20 min and average cell area was plotted over time. Error is SEM (n = 3; *P < 0.05). Scale bar = 40 µm.