Figure S4.
ABI2 and PIM are necessary for proper wound healing. (a) Quantification of the change in cell area per minute upon stimulation with EGF in different oxygen environments. Error = 95% CI (n = 45 5 nM EGF Nx; n = 15, 50 nM EGF Nx; and n = 15, 5 nM EGF Hx for DU145; n = 6 for ABI2-KOA20 in all conditions; *P < 0.05). (b) Wound healing assay with PC3-LN4 cells with a non-targeting siRNA, ABI2-targeting siRNA, or chemical inhibition of PIM. Cell edge at the time of scratch depicted in yellow and edge 8 h post-scratch in blue. Scale bar = 200 µm. (c) Quantification of the scratch assay. Error is SEM (n = 3; *P < 0.05). (d) Western blot depicting efficacy of siRNAs targeting ABI2 or non-specific sequence (negative control). Source data are available for this figure: SourceData FS4.