Figure S5.
Loss of ABI2 does not affect cell proliferation. (a) Cells were plated in 96-well dishes and allowed to grow for 96 h. Wells were stained with Crystal Violet and eluted using SDS. Average absorbance was measured as an indirect measure of the number of cells present and plotted over time. Error is SEM (n = 3; *P < 0.05; color indicates point is significant compared with other cell lines at an indicated time). (b) Graph of relative tumor penetrance representing the proportion of seeded tumor that went on to invade through into the smooth muscle of the diaphragm of A20-DMSO treated mice (n = 4) in relation to DU145-DMSO treated mice (n = 12). Error shown as 95% CI. No significance was observed using two-sided Student’s t test. (c) IHC analysis of Ki67 expression in mouse diaphragms injected with DU145 and ABI2-KOA20 cells with or without overexpression of PIM1. Scale bar = 200 µm.