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. 2023 Apr 13;12(4):13. doi: 10.1167/tvst.12.4.13

Figure 1.

Figure 1.

Standardization of the chromatographic profile for HMWHA, LMWHA, and ULMWHA by size exclusion HPLC. HMWHA, mid-MWHA, and LMWHA were applied to a size exclusion HPLC at increasing concentrations starting from 0.0025 mg/mL to 2.5 mg/mL, and the concentration and molecular weight of HA were successfully determined within a range of 0.014 to 0.25 mg/mL. (A–C) Linear regression equations and r2 were calculated for HMWHA (A), mid-MWHA (B) and LMWHA (C). (D–E) Chromatographic profiles for HMWHA, mid-range MWHA, and LMWHA at increasing concentrations, specifically 0.0025, 0.005, 0.025, 0.05, 0.25, 0.5, 0.75, 1, 1.25, 1.5, 1.75, 2, and 2.5 mg/mL, are shown.