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Identification of HA in HA-containing eye products by analyzing susceptibility to hyaluronidase (Hyalase) digestion. Eight HA-containing eye products were treated with Hyalase or not and then administered to size exclusion HPLC and monitored at 204 nm. An overlay of the chromatographic profiles of the HA-containing eye products that were treated with Hyalase or with enzyme buffer in the absence of Hyalase is presented.
