Table 2.
Identification of the Retention Time and Molecular Weight HA Within the Major Peaks and Concentration of HA Within OTC Eye Products by Size Exclusion HPLC
| Ophthalmic Products | Retention Time (min) | Standard Deviation | Relative Standard Deviation (%) | Molecular Weight of Major HA Peak | Concentration, mg/mL | %, w/v |
|---|---|---|---|---|---|---|
| Biotrue MPS Solution | 14.092 | 0.041 | 0.276 | HMWHA | 0.139 | 0.014 |
| Blink Contacts | 14.974 | 0.050 | 0.351 | Mid-MWHA | 2.898 | 0.184 |
| Blink Gel Tears | 15.254 | 0.042 | 0.272 | Mid-MWHA | 1.843 | 0.403 |
| Blink Tears | 15.333 | 0.041 | 0.281 | Mid-MWHA | 4.032 | 0.290 |
| Clear Eyes Pure Relief | 14.744 | 0.032 | 0.210 | HMWHA | 1.930 | 0.193 |
| CVS Moisturizing Eyelid Spray | No HA detected | — | — | — | — | — |
| CVS Refreshing Eyelid Spray | 14.273 | 0.021 | 0.141 | HMWHA | 0.177 | 0.018 |
| CVS Soothing Eyelid Spray | 16.123 | 0.032 | 0.191 | LMWHA | 1.774 | 0.177 |
| Refresh Repair | 14.437, 15.654 | 0.200 | 1.368 | HMWHA, Mid-MWHA | 0.321 | 0.032 |