TABLE 3.
Fluid or salts exposure | Incubation time (h) | % Survival (mean ± SD) of indicated strain |
||||||
---|---|---|---|---|---|---|---|---|
ATCC BAA-835 | AM01 | AM02 | AM03 | AM04 | AM05 | AM06 | ||
Artificial gastric fluid | ||||||||
pH 3 | 1.5 | 9.77 ± 2.89 | 84.88 ± 10.07 | 15.4 ± 12.02 | 0.18 ± 0.03 | 34.14 ± 1.76 | 10.98 ± 1.26 | 74.97 ± 2.25 |
3 | 2.37 ± 0.58 | 57.75 ± 8.42 | 3.71 ± 1.65 | 0.02 ± 0 | 9.95 ± 1.38 | 1.42 ± 0.11 | 24.4 ± 8.3 | |
pH 2 | 1.5 | 14.42 ± 3.79 | 66.04 ± 18.51 | 5.63 ± 3.06 | 31.45 ± 12.09 | 4.76 ± 1.43 | 2.43 ± 1.02 | 15.56 ± 1.39 |
3 | 9.29 ± 3.22 | 53.20 ± 18.93 | 2.74 ± 1.16 | 3.15 ± 1.21 | 1.87 ± 0.45 | 1.17 ± 0.32 | 10.74 ± 1.17 | |
Artificial intestinal fluid | ||||||||
pH 6.8 | 4 | 82.83 ± 1.86 | 88.13 ± 5.01 | 79.99 ± 11.89 | 84.74 ± 7.78 | 89.35 ± 13.17 | 129.47 ± 11.61 | 102.17 ± 12.17 |
8 | 88.75 ± 9.34 | 107.58 ± 4.83 | 51.03 ± 10.80 | 50.435 ± 5.88 | 124.66 ± 13.14 | 117.08 ± 9.31 | 125.27 ± 29.88 | |
Bile salts | ||||||||
0.5% | 4 | 100.49 ± 10.83 | 87.96 ± 29.70 | 101.25 ± 3.20 | 95.45 ± 8.53 | 92.73 ± 16.38 | 123.85 ± 6.98 | 113.73 ± 12.58 |
8 | 61.83 ± 1.82 | 70.60 ± 9.90 | 76.56 ± 2.41 | 99.47 ± 4.60 | 144.03 ± 47.64 | 112.88 ± 7.91 | 88.47 ± 21.98 | |
1% | 4 | 79.68 ± 5.80 | 65.02 ± 6.25 | 84.93 ± 2.49 | 117.09 ± 9.44 | 114.96 ± 39.2 | 114.58 ± 8.52 | 88.64 ± 7.56 |
8 | 50.57 ± 10.67 | 49.10 ± 2.83 | 62.15 ± 2.31 | 92.83 ± 2.56 | 106.99 ± 20.92 | 80.37 ± 12.02 | 131.31 ± 10.11 |