SLC30A10 expression and brain metal levels of pan-neuronal/glial Slc30a10 knockouts (Slc30a10fl/fl; Nes-Cre) and littermate controls in early postnatal life without Mn exposure. (A) RT-PCR from midbrain (containing the basal ganglia and thalamus) and liver tissue of 4-month-old pan-neuronal/glial Slc30a10 knockouts or littermate controls. (B) RT-PCR from midbrain, liver, and small intestine tissue of a control mouse at PND 8. (C–D) ICP–MS from midbrain containing basal ganglia and thalamus at PND 8 (C) or PND 14 (D). For C, n = 5 controls (2 males, 3 females) and 5 knockouts (3 males, 2 females). For (D), n = 8 controls (3 males, 5 females) and 5 knockouts (all females). Mean ± SE. P > 0.05 by t-test.