Skip to main content
. 2022 Nov 24;15(2):80–91. doi: 10.1111/1758-2229.13133

FIGURE 1.

FIGURE 1

NMDS comparison of microbial communities present in black stains and nearby unstained parts of Lascaux's Apse. Samples from black stains (full symbols) and nearby unstained parts (empty symbols) were taken from the left (red) and right (blue) walls of Lascaux's Apse in June 2015 (circles), June 2016 (triangles) and December 2016 (diamonds), based on the relative proportion of both phyla and classes. (A) Fungal community (ITS2 regions). Differences were found when comparing (i) black stains on the left wall in June 2015 to the same stains at other sampling dates (PERMANOVA F 2,27 = 14.5, p = 0.001, R 2 = 0.31), or (ii) these black stains on the left wall in June 2015 to black stains on the right wall at any of the samplings carried out (June 2016 or December 2016). In contrast, unstained parts next to black stains did not differ between left and right walls. (B) micro‐eukaryotic community (18S rRNA genes). Black stains (but not neighbouring unstained parts) differed between left and right walls (PERMANOVA F 1,30 = 3.8, p = 0.01, R 2 = 0.04), and black stains (but not neighbouring unstained parts) from different sampling dates could differ (PERMANOVA F 2,30 = 7.6, p = 0.001, R 2 = 0.16). (C) Bacterial community (16S rRNA genes). The bacterial community structure differed when comparing left walls to right walls (PERMANOVA F 1,29 = 3.73, p = 0.018, R 2 = 0.04) for black stains (but not for unstained parts), whereas the effect of the sampling date was not significant.