Table 2:

Overview of mother-machine image analysis tools.

Software	Implementation	Segmentation	Deep learning support
BACMMAN [14] / DistNet [39]	ImageJ plugin	2D	✓
DeLTA [11,36]	Python package	2D	✓
napari-MM3 [31], this work	napari plug-in	2D	✓
SAM [35]	MATLAB	2D
MMHelper [13]	ImageJ plugin	2D
MolySo [12]	Python package	1D
MoMa [37]	ImageJ plugin	1D

A comparison of several published imaging methods. ‘2D’ or ‘1D’ segmentation indicates whether the cells are labeled in an image and analyzed in two dimensions, or projected onto a vertical axis and analyzed in one dimension. Several tools support the use of deep learning (as compared to classical computer vision techniques, such as Otsu’s method).