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[Preprint]. 2023 Apr 4:2023.04.03.535447. [Version 1] doi: 10.1101/2023.04.03.535447

Figure 4. Competitive ELISA measuring binding of ACE2 receptor to DCFHP samples in solution and adsorbed to Alhydrogel adjuvant.

Figure 4.

(A) Representative ELISA curves comparing ACE2 binding of DCFHP while in solution and adsorbed to Alhydrogel. (B) Representative ELISA curves of AH-adsorbed DCFHP incubated at 4°, 40°, and 50°C overnight and compared to a DCFHP reference sample that was adsorbed to Alhydrogel on the same day of the assay. (C) Native antigen concentration of thermally-stressed AH-adsorbed DCFHP samples as determined from the ELISA curves (panel B) versus the reference sample. Data are the mean of two independent experiments, which contained two replicates (n=4) with the error bars representing the standard deviation. The dashed line represents the target concentration of 50 mcg/mL. DCFHP samples were at 50 mcg/mL in 20 mM Tris, 150mM NaCl, 5% Sucrose, pH 7.5 buffer, with and without being adsorbed to 1.5 mg/mL Alhydrogel.