Table 1.
Number of CRISPR-Cas9 induced scissions from WGS of surviving TS0111 and Panc10.05 colonies.
| sgRNA | Number of predicted perfect target sites1 | Number of potential off-target sites2 | Number of mutated sites in TS01113 | Number of mutated sites in Panc10.053 | Total number of Cas9-induced cuts in Panc10.055 |
|---|---|---|---|---|---|
| NT | 0 | 0-1 | 0-0-0 | 0-0-0 | 0-0-0 |
| NT2 | 0 | 0-0 | 0-0-0 | 0-0-0 | 0-0-0 |
| HPRTc.80 | 1 | 0-2 | 1-0-0 | 1-0-0 | 1-0-0 |
| HPRTc.465 | 1 | 0-2 | 1-0-0 | 1-0-0 | 1-0-0 |
| 531F(2) | 2 | 4-1 | 2-0-0 | 2-0-0 | 3-0-0 |
| 52F(3) | 3 | 0-0 | 3-0-0 | 3-0-0 | 4-0-0 |
| 715F(5) | 5 | 2-1 | 5-1-04 | 5-1-0 | 9-2-0 |
| 451F(6) | 6 | 0-1 | 6-0-0 | 6-0-0 | 12-0-0 |
| 176R(7) | 7 | 2-1 | 6-1-0 | 6-0-0 | 10-0-0 |
| 551R(8) | 8 | 2-1 | NA | 7-0-0 | 12-0-0 |
| 230F(12) | 12 | 8-1 | NA | NA | NA |
| 164R(14) | 14 | 5-2 | NA | 13-3-04 | 21-5-0 |
| 676F(16) | 16 | 2-6 | 16-1-0 | NA | NA |
1.
Number of perfect matches in CRISPOR using the GRCh38 human reference genome, including both canonical (NGG) and non-canonical (NGA/NAG) PAMs.
2.
From CRISPOR, number of 1 and 2 mismatches (1mm-2mm).
3.
Matched or mismatched sites that are mutated from analysis of two resistant colonies for each sgRNA, using an average variant allele frequency cutoff of 10%. Numbers are shown as 0mm-1mm-2mm.
4.
Only one colony could be obtained.
5.
The number of mutated sites that incorporates copy number of the target for Panc10.05 cell line based on hg19.
NA: not available since no resistant colonies could be obtained.