Table 4.
Gene | Primer Sequences |
---|---|
GAPDH | FP (5’ → 3’): AACCTGCCAAATATGATGAC RP (5’ → 3’): TCATACCAGGAAATGAGCTT |
VCP | FP (5’ → 3’): TGGCATGACTCCCTCCAAAG RP (5’ → 3’): CAGCTCAFFACCCTTGATCG |
45S pre-rRNA | FP (5’ → 3’): GAACGGTGGTGTGTCGTT RP (5’ → 3’): GCGTCTCGTCTCGTCTCACT |
18S rRNA | FP (5’ → 3’): GCCGCTAGAGGTGAAATTCT RP (5’ → 3’): TCGGAACTACGACGGTATCT |
5.8S rRNA | FP (5’ → 3’): GTGGATCACTCGGCTCGTG RP (5’ → 3’): CGCAAGTGCGTTCGAAGTG |
Abbreviations: FP, forward primer; RP, reverse primer, GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VCP, valosin-containing protein.