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[Preprint]. 2023 Apr 6:2023.04.06.535919. [Version 1] doi: 10.1101/2023.04.06.535919

Table 4.

qPCR primer sequences

Gene Primer Sequences
GAPDH FP (5’ → 3’): AACCTGCCAAATATGATGAC
RP (5’ → 3’): TCATACCAGGAAATGAGCTT
VCP FP (5’ → 3’): TGGCATGACTCCCTCCAAAG
RP (5’ → 3’): CAGCTCAFFACCCTTGATCG
45S pre-rRNA FP (5’ → 3’): GAACGGTGGTGTGTCGTT
RP (5’ → 3’): GCGTCTCGTCTCGTCTCACT
18S rRNA FP (5’ → 3’): GCCGCTAGAGGTGAAATTCT
RP (5’ → 3’): TCGGAACTACGACGGTATCT
5.8S rRNA FP (5’ → 3’): GTGGATCACTCGGCTCGTG
RP (5’ → 3’): CGCAAGTGCGTTCGAAGTG

Abbreviations: FP, forward primer; RP, reverse primer, GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VCP, valosin-containing protein.