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. 2023 Apr 14;9(15):eadf2695. doi: 10.1126/sciadv.adf2695

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Fig. 6. — (A to D) Genotyping of LSL-DdCBE-Nd2 KI (A), LSL-DdCBE-Nd5 KI (B), LSL-DdCBE-Cytb KI (C), and LSL-DdCBE-Cox3 KI (D) F₀ rats by PCR with upstream primer pair (Up band), downstream primer pair (Down band), and internal primer pair (DddA_tox band). (E and F) The recombination sites were confirmed by Sanger sequencing for upstream primer pair (E) and downstream primer pair (F) in LSL-DdCBE-Nd2 KI, LSL-DdCBE-Nd5 KI, LSL-DdCBE-Cytb KI, and LSL-DdCBE-Cox3 KI rats. (G) Six cKO rat strains covering four respiratory chain complexes were generated in this study.