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. 2023 Apr 14;12:e76182. doi: 10.7554/eLife.76182

Figure 3. F. perrara ihfA* mutant displays a colonization defect.

(A) Light microscopy pictures of pylorus region of bees colonized with F. perrara PEB0191wt or ihfA* 10 d post colonization. (B) Quantification of scab phenotype of bees 5 and 10 d post colonization with n = 18 and n = 36 per treatment, respectively. (C) Quantification of colonization levels is measured by colony-forming units (CFUs) at day 5 (n = 18) and day 10 (n = 36) post colonization. Wilcoxon rank-sum test was used to assess significant differences. (D) Time-course experiment of bees colonized with F. perrara wt or ihfA*. Colonization levels were measured by CFUs every second day until day 10 and then at day 14 and day 21. n = 12 bees per time point per treatment. Wilcoxon rank-sum test was used to assess significant differences per time point. Error bars represent median and interquartile range. Data from three independent experiments. *p<0.05, **p<0.01, ***p<0.001. Figure 3—source data 1 contains the numeric values for the figures shown here.

Figure 3—source data 1. Numeric data underlying the results shown in Figure 3 and Figure 3—figure supplement 1.

Figure 3.

Figure 3—figure supplement 1. Quantification of F. perrara wt and ihfA* in the pylorus and ileum.

Figure 3—figure supplement 1.

Mono-colonization of bees with F. perrara wt and ihfA*. Colonization levels are measured by colony-forming units (CFUs) and quantitative PCR (qPCR) 10 d post colonization in the specified gut regions: pylorus and ileum. Wilcoxon rank-sum test was used to assess the statistical significance of differences. n = 6. Figure 3—source data 1 contains the numeric values for the figure shown here.