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. 2023 Apr 14;12:e76182. doi: 10.7554/eLife.76182

Figure 5. Gene expression of Ihf-regulated genes of F. perrara 10 d post inoculation of gnotobiotic honey bees.

Transcripts per million were calculated for all replicates of the in vitro and the in vivo RNAseq experiments. For the in vitro experiment (Exp. 1), all three replicates of the wt and the ihfA* mutant are shown. For the in vivo experiment (Exp. 2), the four replicates of the day 10 time point and the in vitro reference condition are shown. Data for day 5 time point in comparison to day 10 time point is shown in Figure 5—figure supplement 2. Figure 5—source data 1 and Figure 5—source data 2 contain the data used to generate the figure shown here. (A-F) The expression of Flp pili genes (A), genes involved in the synthesis of Arylpolyene (B) and Colibactin (C) and genes involved in the function of T6SSs (D-F) is shown.

Figure 5—source data 1. List of F. perrara genes differentially expressed in vivo.
elife-76182-fig5-data1.xlsx (271.2KB, xlsx)
Figure 5—source data 2. Clusters of orthologous groups for genes differentially expressed in vivo.

Figure 5.

Figure 5—figure supplement 1. RNAseq comparison of F. perrara during in vivo colonization compared to growth in vitro.

Figure 5—figure supplement 1.

Significantly differentially expressed genes with a |log2-fold change| >|2| of F. perrara PEB0191 wt at day 5 (A) and day 10 (B) post colonization in comparison to growth in vitro. Genes are colored according to their category. The experiment was performed in quadruplicates, all genes displayed were significantly differentially expressed with p<0.05 and a false discovery rate (FDR) < 0.05 (exact test).
Figure 5—figure supplement 2. Gene expression of Ihf-regulated genes of F. perrara 5 d post inoculation of gnotobiotic honey bees.

Figure 5—figure supplement 2.

Same as Figure 5, but only time point day 5 and day 10 are depicted in comparison to F. perrara ihfA* in vitro. (A-F) The expression of Flp pili genes (A), genes involved in the synthesis of Arylpolyene (B) and Colibactin (C), and genes involved in the function of T6SSs (D-F) are shown.