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. 2023 Mar 31;14:1130933. doi: 10.3389/fimmu.2023.1130933

Figure 1.

Figure 1

A model for ILC2-specific genetic ablation of ST2. (A), Expression of the Il1rl1 gene in Il1rl1 flox/flox (Ctrl) and Nmur1 iCre-eGFP Il1rl1 flox/flox (cKO) sort-purified ILC2s. (B), Flow cytometric histograms of ST2 expression in ILC2s of Il1rl1 flox/flox (Ctrl) and Nmur1 iCre-eGFP Il1rl1 flox/flox (cKO) mice across organs, including isotype control on Il1rl1 flox/flox (Iso) in the steady state. ILC2s were gated on live CD45+ Lin- (CD3, CD5, CD19, Ly6G, Fcϵr1), CD127+ and KLRG1+. (C–G) Percentage of ST2+ expression in Il1rl1 flox/flox (Ctrl), Nmur1 iCre-eGFP Il1rl1 flox/flox (cKO) (D–G) and Il1rl1 -/- (ST2 KO) mice including isotype control (C) across different organs in the indicated immune cells. Basophils from spleen and mast cells from the peritoneal lavage. For (C–G): Each symbol represents data from one mouse, data are representative of two experiments with four to six mice per group. Mean +/- SD, Student’s t-Test. ns, not significant, **p < 0.01, ****p < 0.0001.