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. 2023 Feb 10;10(11):2204882. doi: 10.1002/advs.202204882

Figure 5.

Figure 5

MAPS+CD11c+ cells migrated from the implant sites to draining lymph nodes and the spleen. a) MFI of AF647 among all CD11c+ live cells infiltrating the implants, residing in dLN or spleen. b) Pie charts of APC cell abundancy across 3 time points for both D‐MAPS and L‐MAPS [NK and T cells (CD3+), B cells (pDCA‐1− B220+), pDC (pDCA‐1+ B220+), neutrophil (CD11b+ Ly6G+), macrophage (Ly6C+, CD169+, F4/80+), moDC, monocytes, and MCM (CD64+ CD11b+) monocyte‐derived DC CD64− cDCs resident (CD11chi I‐A/I‐Eint CCR7int), cDCs migratory (CD11cint I‐A/I‐Ehi CCR7hi), cDC1 (XCR1hi CD172alo), and cDC2 (XCR1lo CD172ahi)]. c) The percentages of dendritic cell subtypes cells among all CD45+ live cells infiltrating the implants across 3 different time points. d) The percentages of CCR7 high migratory DC and resident DC among all DCs on day 4, day 7, and day 14. e) The percentages of cDC1 and cDC2 among all DCs on day 4, day 7, and day 14. f) MFI of MHCII, CCR7, CD80, and ICAM‐1 in pDC, moDC, and cDC on day 7. g) The percentages of T and NK‐T cells, B cells among all CD45+ live cells infiltrating the implants across 3 different time points. Statistical analysis: two‐way ANOVA with Šídák's multiple comparisons test made between L‐ and D‐MAPS groups only when there was a significance in the interaction term of scaffold type x time. In panel (a), after a two‐way ANOVA, Dunnet method was used to compare the experimental groups with the baseline control group. * p<0.05, **/## p<0.01, ***/### <0.001, ****/#### <0.0001. Asterisks stand for comparisons between L‐ and D‐MAPS. Pound signs stand for comparisons between L‐ or D‐MAPS and the baseline control (mice without implant). Error bars, mean ± s.e.m. n = 6 mice per group with some data points removed due to experimental reasons. The purple symbol in the bottom left corner of the graph stands for the 20‐color APC panel used in this figure. pDC, plasmacytoid dendritic cell. moM, monocyte‐derived macrophage. moDC, monocyte‐derived dendritic cell (activation/maturation (MHCII), co‐stimulation (CD80), and adhesion (ICAM‐1)).