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. 2023 Jan 31;10(11):2206344. doi: 10.1002/advs.202206344

Figure 8.

Figure 8

Necroptotic T cell contributes to increased susceptibility to colitis in RPA1‐deficient mice. a) Body weight of 6‐week‐old male WT and CKO mice with or without CD8+ T cell depletion treated with 2% (weight/volume) DSS (WT‐control, n = 7 mice; CKO‐control, n = 6 mice; WT‐CD8+ T depletion, n = 7 mice; CKO‐CD8+ T depletion, n = 6 mice; mean ± s.e.m., ***P < 0.001, two‐tailed unpaired Student's t‐test). b) Survival analysis of 6‐week‐old male WT and CKO mice with or without CD8+ T cell depletion treated with 2% (weight/volume) DSS (WT‐control, n = 7 mice; CKO‐control, n = 6 mice; WT‐CD8+ T depletion, n = 7 mice; CKO‐CD8+ T depletion, n = 6 mice; *P = 0.0122, Log‐rank (Mantel‐Cox) test). c‐d) Macroscopic evaluation c) and colon length d) of WT and CKO mice with or without CD8+ T cell depletion treated with 2% (weight/volume) DSS for 6 days (n = 4 mice; mean ± s.e.m., **P = 0.0032, ns, no significant (P > 0.05), two‐tailed unpaired Student's t‐test). e) Body weight of 6‐week‐old male WT and CKO mice treated with 2% (weight/volume) DSS with or without GSK872 (2 mg/kg) every other day (WT, n = 3 mice; WT+GSK872, n = 5 mice; CKO, n = 3 mice; CKO+GSK872, n = 5 mice; mean ± s.e.m., ns, not significant (P > 0.05), **P = 0.0064, ***P = 0.0007, two‐tailed unpaired Student's t‐test). f–g) Macroscopic evaluation f) and colon length g) of WT and CKO mice treated with 2% (weight/volume) DSS for 6 days with or without GSK872 (2 mg kg−1) every other day (n = 3, mean ± s.e.m., ns, not significant (P > 0.05), **P = 0.0016, ***P = 0.0006, two‐tailed unpaired Student's t‐test). h) Flow cytometric analysis of the frequencies of macrophage, neutrophil, pDC, cDC, CD4+ T cells, CD8+ T cells from colon in WT or CKO mice treated with DSS in presence or absence of GSK872 (n = 5 biological replicates, mean ± s.e.m., ns, not significant (P > 0.05), *P < 0.05, **P < 0.01, two‐tailed unpaired Student's t‐test). i) Immunoblot analysis of protein levels of phosphorylated MLKL, phosphorylated RIPK3, MLKL and RIPK3 in CD8+ T lymphocytes derived from 6‐week‐old WT and CKO mice treated with DSS in presence or absence of GSK872 (n = 3 biological replicates).