Figure 9.

CKO mice are more sensitive to autoimmune hepatitis. a) Survival analysis of 6‐week‐old male WT and CKO mice treated with 30 mg kg⁻¹ Con A (n = 10 mice; **P = 0.0019, Log‐rank (Mantel–Cox) test). b) 6‐week‐old male WT and CKO mice were intravenously injected with 30 mg kg⁻¹ Con A and serum was collected 8 hours after injection. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were measured by Biochemical analyzer (Control, n = 4 mice; Con A, n = 5 mice; mean ± s.e.m., *P = 0.0263, **P < 0.01, two‐tailed unpaired Student's t‐test). c–e) Flow cytometric analysis of the frequencies of CD8⁺ T cells, CD4⁺ T cells and CD25⁻CD69⁻ cells, CD25⁻CD69⁺ cells and CD25⁺CD69⁺ cells in CD8⁺ T cells or CD4⁺ T cells derived from liver of WT and CKO mice treated with Con A for 8 h (n = 5, mean ± s.e.m., ****P < 0.0001, two‐tailed unpaired Student's t‐test). f–g) Flow cytometric analysis of the frequencies of B220⁺ cells and CD11b⁺ cells derived from liver of WT and CKO mice treated with Con A for 8 h (n = 5, mean ± s.e.m., ****P < 0.0001, two‐tailed unpaired Student's t‐test). h) Representative immunohistochemistry staining pictures of CD3ε and S100A8/S100A9 protein levels in liver tissues from WT and CKO mice treated with Con A for 8 h (scale bars represent 200 µm).