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. 2022 Mar 25;30(4):567–574. doi: 10.1038/s41417-022-00450-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Cell viability assay showing Len-sensitive MM1S and Len-resistant MMR10R cell line treated with Lenalidomide at indicated concentrations. IC₅₀ values were calculated by GraphPad Prism 8. B Western blot showing CRBN, IRF4, SAE2, SAE1, UBC9, and global SUMOylation (SUMO-2,3 and SUMO-1) level of MM1S and MMR10R cell lines; GAPDH, loading control. Relative protein level was quantified using Image J, normalized to GAPDH, and labeled below each blotting band.