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. 2022 Mar 25;30(4):567–574. doi: 10.1038/s41417-022-00450-9

Fig. 2. SUMOylation inhibition synergizes with Len in decreasing cell viability in both Len-sensitive and Len-resistant MM cell lines.

Fig. 2

A TAK-981 synergizes with Len cytotoxicity in sensitive MM line (MM1S) and resistant MM line (MMR10R). MM1S and MMR10R cells were treated with indicated concentration of TAK-981 or Len or both (TAK + Len) with indicated concentration for 48 h and cell viability was determined by Cell-Titer-Glo. Drug synergy was analyzed using CompuSym program. Simulating calculated CI values (open circle) and experimental combination indice (CI) values (solid circle) based on combination data points are plotted as a function of the fractional affected (Fa) derived from analysis report. Fraction affected indicates percentage inhibition of cell growth/100. Drug synergism is defined as CI < 1. B TAK-981 enhances cytotoxicity of Len in sensitive and resistant MM. MM1S and MMR10R cells were treated with Vehicle (Veh), 0.1 µM TAK-981 (TAK), 2.5 µM Len (Len), or 0.1 µM TAK-981 with 2.5 µM Len (TAK + Len). Apoptosis was measured by flow cytometry using Annexin V/PI staining. C Quantified apoptosis from three experimental repeats. Data were analyzed using one-way ANOWA test: Data presented as mean ± SD. *p < 0.05; **p < 0.01; ****p < 0.0001.