Extended Data Fig. 7. SARS-CoV-2 infection induces senescence in hACE2-mouse lungs.
A) Mouse body weight was monitored daily for 7 days and expressed as the percentage relative to day -1. Single values of PBS-control mice (black dots) and infected mice (red dots) are represented. B) Quantification of SARS-CoV-2 RNA in the lung of PBS-treated control (n = 3) and SARS-CoV-2 infected mice (n = 3) measured at 6 days post-infection. Values are expressed as copy number per ng of total RNA. C) IHC images of sections (n = 3) of the lungs from irradiated (IR, n = 1) or not irradiated (NI, n = 1) wild-type mice analyzed as controls of DDR activation. Scale bar, 20 μm. D) Quantification of DDR activation represented in C, each dot corresponds to a section. E) IHC images of lungs from SARS-CoV-2 infected hACE2-mice or mock-infected (PBS) wild-type mice probed for p16 or p21. Scale bar, 50 μm. F) Quantification of the percentage of cells positive for the markers shown in E, each dot corresponds to a mouse. G) IF images of a representative experiment of lungs, from SARS-CoV-2 infected hACE2-mice probed for the indicated proteins. CD68 and prosurfactant protein C (proSP-C) markers were used to label macrophages and pulmonary alveolar type II cells, respectively. Scale bar, 50 μm. The experiment was repeated three times with similar results. Source numerical data are available in source data.