Fig. 2. SARS-CoV-2 reduces CHK1 and RRM2 levels leading to dNTP shortage.
a, Immunoblotting of whole cell lysates of Huh7 infected, or not, with SARS-CoV-2 and analysed at different timepoints post-infection. b, Quantification of protein levels shown in a; values are shown as relative to mock-infected samples. c, Immunofluorescence (IF) images of infected (V+) or mock-infected (V−) Huh7 cells fixed 48 h post-infection; nuclei were stained with DAPI. Scale bar, 10 μm. d, Quantification of CHK1- or RRM2-positive cells shown in c; n = 3 independent experiments. e, dNTP concentration was measured in V− or V+ Huh7 and Calu-3; values are shown as relative to V−. f, Histograms show the percentage of cells in each phase of the cell cycle in V− or V+ Huh7 fixed 48 h post-infection. g, Fraction of V− or V+ Huh7 cells that did not incorporate BrdU (BrdU−) measured by flow cytometry 48 h post-infection. Source numerical data and unprocessed blots are available in source data.