Fig. 3. dN supplementation is sufficient to reduce DNA damage and inflammation.
a, Immunofluorescence (IF) images of V− or V+ Huh7 cells, treated or not with dNs, fixed 48 h post-infection; nuclei were stained with DAPI. Scale bar, 10 μm. b, Quantification of DDR activation shown in a. Each dot is a nucleus. c, Immunoblots of Huh7 cells treated as in a. d, Quantification of protein levels shown in c. Values are normalized to untreated V+ cells. e, Images of comet assays of Huh7; conditions are as in a. Scale bar, 100 μm. f, Quantification of comet tail moment shown in e; horizontal bars represent the median values ± 95% CI of three independent infections. g, RT–qPCR of pro-inflammatory cytokine expression in V− or V+ Calu-3 cells, treated or not with dNs. Values are shown as relative to RPLP0 mRNA. Source numerical data and unprocessed blots are available in source data.