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. 2023 Apr 14;13:6118. doi: 10.1038/s41598-023-32854-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell-based NanoBRET assays with TTBK lead inhibitors. (A) Structure of tracer 29. (B) Competition experiments between tracer 29 and analog 3, 9, or 10 at either 10 or 30 µM for NLuc-TTBK1 and NLuc-TBK2, n = 1. (C) Tracer titration results for NLuc-TTBK1 and NLuc-TTBK2 in the presence of analog 3, n = 1. Calculated IC₅₀ values for 3 are shown for each kinase at the recommended working tracer concentration of 2 µM. (D) Representative NanoBRET assay curves and corresponding IC₅₀ values for active compounds AMG28, 3, 9, and 10 in intact HEK293 cells, n = 2. Error is shown and reported as standard error mean (SEM).