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. 2023 Apr 14;13:6118. doi: 10.1038/s41598-023-32854-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Images and quantification of cilia following treatment of human iPSCs with 9 or 10. (A) Visualization of cilia on human iPSCs following 72 h continuous starvation and treatment with DMSO or 1 μM of either 9 or 10 at 40 × magnification. Arrows are used to highlight the location of some cilia in each panel and a legend of the stains used to visualize cilia and nuclei is placed above the panels. Primary cilia are labeled with the Arl13b antibody (green) and the nuclei with DAPI (blue). (B) Quantification of the percentage of ciliated cells per single field in (A). Error bars represent the SEM. (C, D) Quantification of the percentage of ciliated cells averaged from six random visual fields following 24 h (C) or 72 h (D) of continuous treatment plus starvation and in the presence of different concentrations of 9 or 10. Error bars represent SEM.