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. 2023 Apr 14;14(4):271. doi: 10.1038/s41419-023-05803-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Western blot analysis of RKO, HCT116 and HT29 cells expressing empty vector (EV) or Flag-CYP1B1. B Western blot analysis of RKO, HCT116, and HT29 cells expressing control (ctrl) shRNA or CYP1B1 shRNA. C, D Viability curves of RKO, HCT116 and HT29 cells with CYP1B1 overexpression or knockdown treated with the indicated concentrations of RSL3. E–H Lipid peroxidation levels were tested using C11-BODIPY in CYP1B1 overexpression (E, F) or knockdown (G, H) RKO cells. The data shown represents the mean ± SD; n = 3. p value was determined by Student’s two-sided t test. **p < 0.01, ***p < 0.001.