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. 2023 Apr 14;14(4):271. doi: 10.1038/s41419-023-05803-2

Fig. 4. CYP1B1 expression attenuates the effect of anti-PD-1 therapy.

Fig. 4

A Western blot analysis of MC38 cells expressing ctrl shRNA or CYP1B1 shRNAs. B C57BL/6 J mice carrying the indicated MC38 cell formed tumors at the right flank were treated with anti-mPD-1 antibody or isotype control IgG for a total of five treatments. C, D Tumor images C and tumor growth curve D in mice bearing indicated MC38 cell tumors treated with anti-mPD-1 or isotype control IgG antibodies. E IHC staining of 4-HNE in MC38 tumors expressing ctrl or CYP1B1 shRNA. F Quantification of 4-HNE IHC staining. n = 5 per group. p value was determined by Student’s two-sided t test. *p < 0.05, **p < 0.01, ***p < 0.001.