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. 2023 Apr 14;13:6126. doi: 10.1038/s41598-023-33151-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PTGS2/PGE2 signaling pathway affected CSE-induced ferroptosis in BECs. (A) Screening of DEGs. Red dots are differentially expressed mRNAs, and blue dots are stable genes. (B) Venn diagrams of DEGs and ferroptosis-related genes. (C) The cluster heatmap visualization of ferroptosis-related differential genes (drawn with R language). (D) qRT-PCR verification of PTGS2, HMOX1, and MT1G expression. (E) PTGS2 expression was determined by qRT-PCR and western blot. (F) ELISA detection of PGE2, and lipid peroxidation MDA and SOD. (G) The content of catalytic Fe (II) was detected by FCM. (H) The protein expression of ferroptosis markers TfR, FtL, and GPX4. *P < 0.05 vs. Control, #P < 0.05 vs. CSE, &P < 0.05 vs. CSE + NC. The data were presented in the form of mean ± standard deviation. n = 3.

PTGS2/PGE2 signaling pathway affected CSE-induced ferroptosis in BECs. (A) Screening of DEGs. Red dots are differentially expressed mRNAs, and blue dots are stable genes. (B) Venn diagrams of DEGs and ferroptosis-related genes. (C) The cluster heatmap visualization of ferroptosis-related differential genes (drawn with R language). (D) qRT-PCR verification of PTGS2, HMOX1, and MT1G expression. (E) PTGS2 expression was determined by qRT-PCR and western blot. (F) ELISA detection of PGE2, and lipid peroxidation MDA and SOD. (G) The content of catalytic Fe (II) was detected by FCM. (H) The protein expression of ferroptosis markers TfR, FtL, and GPX4. *P < 0.05 vs. Control, #P < 0.05 vs. CSE, &P < 0.05 vs. CSE + NC. The data were presented in the form of mean ± standard deviation. n = 3.