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. 2022 Nov 2;44(5):1014–1028. doi: 10.1038/s41401-022-01010-5

Fig. 2. The ferroptosis inhibitor LPT1 decreases steatosis and mitochondrial ROS in a mouse MAFLD model.

Fig. 2

a Oil red O staining showing the lipid droplets (red) in liver tissues of four groups of mice. b Liver triglycerides levels in liver tissues of four groups of mice. n = 6 biologically independent experiments. c Liver cholesterol levels in liver tissues of four groups of mice. n = 6 biologically independent experiments. d–g Representative immunoblot analysis of p-Akt, Akt, p-IRS1, IRS-1 and PPARα in liver tissues from four groups of mice. GAPDH was used as loading control. h–l The mRNA levels of lipid oxidation gene Pparα and lipid synthesis genes including Scd1, Fasn, Hmgcr and Cpt1a in liver tissues of four groups of mice. n = 4–6 biologically independent experiments. m Mitochondrial ROS content measured by MitoSox Dye (red) in liver tissues of four groups of mice. Hoechst 33342 (blue) was used to label nuclei. n = 9 biologically independent experiments. The data were presented as Means ± SEM and analyzed by One way-ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01 vs Chow; #P < 0.05, ##P < 0.01 vs HFHF. NS no significance.