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. 2022 Nov 2;44(5):1014–1028. doi: 10.1038/s41401-022-01010-5

Fig. 4. The ferroptosis inhibitor LPT1 suppresses steatohepatitis and ‘PANoptosis’ in a mouse MAFLD models.

Fig. 4

a, b The mRNA level of pro-inflammatory factor TNF-α and IL-1β in liver tissues from four groups of mice. n = 6 biologically independent experiments. c TUNEL staining showing apoptotic cells in liver tissues from four groups of mice. The arrows show TUNEL positive cells (brown). Scale bars, 100 μm. d–f Representative immunoblot images and quantitative analysis on phosphorylated MLKL (p-MLKL), total MLKL, Bax (a pro-apoptotic protein) and Bcl-xL (an anti-apoptotic protein) protein levels in liver tissues from four groups of mice. GAPDH as loading control. g The enzymatic activity of pyroptosis executor Caspase-1 in liver tissues from four groups of mice. n = 5 biologically independent experiments. h The mRNA level of pyroptosis executor Gsdmd in liver tissues from four groups of mice. n = 4 biologically independent experiments. i–k Representative immunoblot images and quantitative analysis on the cleavage of Caspase-1 and GSDMD in liver tissues from four groups of mice. GAPDH as loading control. l–n The mRNA level of IL-6, IL-1β and TNF-α in liver tissues from four groups of mice fed with MCD or normal chow diet for 4 weeks. n = 6 biologically independent experiments. The data were presented as Means ± SEM and analyzed by One way-ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01 vs Chow; #P < 0.05, ##P < 0.01. NS no significance.