Fig. 9. An iron chelator DFP does not block PANoptosis.

a Representative immunoblot images and quantitative analyses of p-MLKL, MLKL, GSDMD and Caspase-6 in liver tissues in three groups of mice. GAPDH as loading control. n = 3 biologically independent experiments. b Serum ALT and AST levels in three groups of mice which were induced by HFHF diet for 16 weeks. DFP was given by oral gavage (100 mg·kg⁻¹·d⁻¹) for two weeks. c A schematic representation depicting the different impacts of ferroptosis inhibitor (radical trapping antioxidant) and iron chelator on steatosis and steatohepatitis in MAFLD condition. Ferroptosis inhibitor not only inhibits ferroptosis, but also blocks apoptosis, pyroptosis and necroptosis (PANoptosis). By contrary, iron chelator does not affect PANoptosis process. The data were presented as Means ± SEM and analyzed by One way-ANOVA followed by Tukey’s post hoc. **P < 0.01 vs Chow. NS no significance.