Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 14;14:2144. doi: 10.1038/s41467-023-37761-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Quantification of percent UTs co-localized with p-RPA32 (S33) in Rap1^−/− MEFs expressing TRF2^∆B. Data represents the mean of three independent experiments ± SD from a minimum 200 nuclei analyzed per experiment. ****P < 0.0001 by one-way ANOVA. ns non-significant. b Quantification of percent UTs co-localized with RAD51 in Rap1^−/− MEFs expressing TRF2^∆B. Data represents the mean of three independent experiments. At least 200 nuclei were examined per experiment. ****P < 0.0001 by one-way ANOVA. ns non-significant. c Quantification of UT frequencies in the absence of NBS1 and Ligase 3 that promote A-NHEJ. Data represents the mean values from two independent experiments ± SD from a minimum 200 nuclei analyzed per experiment. ***P = 0.0005, ****P < 0.0001 by one-way ANOVA. ns non-significant. d Quantification of the percentage of U2OS cells possessing UTs with or without treatment with SLX4 shRNA. Data represents the mean of three independent experiments ± SD from a minimum 200 nuclei analyzed per experiment. ****P < 0.0001 by one-way ANOVA. ns non-significant. e IF-FISH for HA-POLθ (green) co-localizing with telomeres (red) in U2OS cells expressing TRF2^ΔB,L288R. Scale bars: 5 µm. f Quantification of UTs in U2OS cells expressing the indicated DNA constructs. Data represents the mean of three independent experiments ±SD from a minimum 150 nuclei analyzed per experiment. *P = 0.0295, **P = 0.0051, ****P < 0.0001 by one-way ANOVA. ns non-significant. g Quantification showing average telomere size per nucleus in U2OS, Rap1^–/– and Rap1^+/+ MEFs expressing indicated DNA constructs. Data represents the mean of three independent experiments ±SD from a minimum 100 nuclei analyzed per experiment. *P = 0.01, **P = 0.0064, ****P < 0.0001 by one-way ANOVA. ns non-significant. Source data are provided as a Source Data file.