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. 2023 Apr 14;14:2144. doi: 10.1038/s41467-023-37761-w

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© The Author(s) 2023, corrected publication 2023

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Fig. 3 — a Quantification of UT frequencies in ATRX-positive and ATRX-null ALT cells expressing indicated DNA constructs. Data represents the mean of two independent experiments ±SD from a minimum 200 nuclei analyzed per experiment. ***P = 0.0009, ****P < 0.0001 by one-way ANOVA. b Quantification of percent UTs co-localized to PML bodies in U2OS expressing shTRF2 + TRF2^ΔB;L288R. Data represents the mean of three independent experiments ±SD from a minimum 150 nuclei analyzed per experiment. *P = 0.0460, ****P < 0.0001 by one-way ANOVA. c Representative IF-FISH images showing the loss of ATRX promotes the formation of UTs colocalized with PML (green) in Rap1^–/– MEFs expressing TRF2^ΔB. Scale bars: 5 µm. d Quantification of cells containing ≥4 UTs with or without Atrx shRNAs in (c). Data represents the mean of three independent experiments ± SD from a minimum 250 nuclei analyzed per experiment. *P = 0.0407, **P = 0.0028, ***P = 0.0009, ***P < 0.0001 by one-way ANOVA. ns: non-significant. e Quantification of percent UTs co-localized to PML bodies in (c). Data represents the mean of three independent experiments ± SD from a minimum 250 nuclei analyzed per experiment. ****P < 0.0001 by one-way ANOVA. ns non-significant. f Quantification of cells containing ≥4 UTs with or without RAD52 shRNAs. The mean of three independent experiments ± SD shown from a minimum 200 nuclei analyzed per experiment. ***=0.0008 by one-way ANOVA. ns non-significant. Source data are provided as a Source Data file.