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. 2023 Apr 14;14:2144. doi: 10.1038/s41467-023-37761-w

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a IF-FISH showing lamin A staining (green) in Rap1^–/– MEFs expressing indicated constructs and upon expressing WT lamin A or Progerin. Scale bars: 5 µm. b Quantification of UT frequencies in (a). Data represents the mean of three independent experiments ± SD from a minimum 400 nuclei analyzed per experiment. **P = 0.0036, ****P < 0.0001 by one-way ANOVA. ns: non-significant. c Quantification of NE rupturing in cells expressing UTs in Rap1^–/– MEFs expressing indicated constructs. Data represents the mean of three independent experiments ± SD from a minimum 400 nuclei analyzed per experiment. ****P < 0.0001 by one-way ANOVA. ns: non-significant. d Quantification of UT frequencies in Lmna^+/+ and Lmna^–/– MEFs treated with shTrf2 + TRF2^ΔB;L286R. Data represents the mean of three independent experiments ± SD from a minimum 300 nuclei analyzed per experiment. ***P = 0.0008, ****P < 0.0001 by one-way ANOVA. ns non-significant. e IF-FISH showing the impact of CDK1 and ATR phospho- and dephospho-GFP-tagged Lamin mutants (green) in the generation of UTs in U2OS cells expressing TRF2^∆B;L288R. Scale bars: 5 µm. f Quantification of UT frequencies in (e). Data represents the mean of three independent experiments ± SD from a minimum 350 nuclei analyzed per experiment. *P = 0.0246, ***P = 0.0001 ****P < 0.0001 by one-way ANOVA. ns non-significant. g Quantification of UT frequencies in U2OS cells expressing indicated constructs treated with ATR inhibitors (ATRi). Data represents the mean of two independent experiments ± SD from a minimum 300 nuclei analyzed per experiment. *P = 0.0204, **P = 0.0031, ***P = 0.001, ****P < 0.0001 by one-way ANOVA. ns non-significant. h Representative IF-FISH showing that 4.0 µM Ro-3306 CDK1 specific inhibitor (CDKi) reduced the formation of UTs but not p-RPA32(S33) (green) TIFs in Rap1^−/− MEFs expressing indicated proteins. i Quantification of UTs in Rap1^–/– MEFs and U2OS cells in the absence and presence of CDK1 inhibitor. Data represents the mean of two independent experiments ± SD from a minimum 300 nuclei analyzed per experiment. **P = 0.0023, ****P < 0.0001 by one-way ANOVA. ns non-significant. Scale bars: 5 µm. Source data are provided as a Source Data file.