Fig. 1.

Circ_0011385 expression was upregulated in CRC tissues and cells. (A) The differently expressed circRNAs were analyzed by GSE126094 dataset in CRC and normal tissues. (B) CircBase online database was employed to present the genetic messages of circ_0011385. (C) The presence of circ_0011385 was confirmed by qRT-PCR, followed by Sanger sequencing. (D and E) QRT-PCR was conducted to detect the expression level of circ_0011385 in 50 pairs of CRC, paracancerous normal tissues and NCM460, LOVO, SW620, SW480, HCT116 cells. (F and G) RNase R resistance analysis of circ_0011385 assay was carried out to illustrate circ_0011385 was a circular RNA. (H and I) The expression levels of circ_0011385 and EIF3I mRNA were determined by qRT-PCR at 0 h, 6 h, 12 h, 18 h and 24 h, respectively, after actinomycin D treatment in HCT116 and SW480 cells. ∗p < 0.05.