Fig. 6.

MYO6 silencing suppressed CRC progression. (A and B) The impacts of MYO6 silencing on the mRNA and protein levels of MYO6 were determined by qRT-PCR and Western blot, respectively, in HCT116 and SW480 cells. (C–E) MTT and cell colony formation assays were employed to determine the effect of MYO6 knockdown on the proliferation of HCT116 and SW480 cells. (F, I and J) Flow cytometry analysis was conducted to disclose the influences of MYO6 absence on the apoptosis and cell cycle of HCT116 and SW480 cells. (G and H) Wound-healing and transwell invasion assays were performed to reveal the effects of MYO6 silencing on the migration and invasion of HCT116 and SW480 cells, respectively. (K and L) The influences of MYO6 knockdown on the protein expression of CDK1 and Cyclin D1 were presented by Western blot analysis in HCT116 and SW480 cells. ∗P < 0.05.