Fig. 2.

The properties ofmousec-Ddx. (A), Abundance of c-Ddx expression determined by quantitative PCR in various adult mouse tissues,*P < 0.05 vs. SM, n = 3 per group; (B), A time course of myocardial expression levels of c-Ddx in response to swimming training or sedentary life in mice, swimming was terminated at 3 weeks, *P < 0.05 vs. Sed group, n = 6 per group at each time point; (C), Abundances of c-Ddx and glyceraldehyde-3-phosphate dehydrogenase (GADPH) in adult mouse myocardial tissue treated with RNase R, *P < 0.05 vs. Mock group, n = 3 per group; (D), Divergent primers amplify c-Ddx of adult mouse heart in complementary DNA but not in genomic DNA, divergent and convergent primers are indicated by the directions of the arrows; (E), Abundance of nuclear and cytoplasmic c-Ddx in NMCMs, *P < 0.05 vs. Cytoplasm group, n = 3 per group; (F), RNA fluorescence in situ hybridization for c-Ddx in NMCMs (bar = 5 µm); Abbreviations: c-Ddx, circ-Ddx60; EHP, exercise hypertrophic preconditioning; NMCMs, neonatal mouse cardiomyocytes; Sed, sedentary; SM, skeletal muscle; LV, left ventricular tissue;