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. 2022 Jun 16;46:113–121. doi: 10.1016/j.jare.2022.06.005

Fig. 4.

Fig. 4

Effect of silencing of c-Ddx on myocardial hypertrophy, cardiac fibrosis and pulmonary congestion in EHP mice with pressure overload. (A), Representative mouse hearts (scale bar = 2 mm); (B), HW:BW ratio and HW:TL ratio; (C), Representative myocardial cross-sections stained with wheat germ agglutinin (scale bar = 25 µm) and quantization of cardiomyocyte cross-sectional area; (D), Representative myocardial cross-sections stained with masson trichrome (scale bar = 50 µm) and quantization of myocardial fibrosis; (E), LW:BW ratio; (F), LW:TL ratio; (G), Silencing of c-Ddx blocked the EHP-induced downregulation of Myh7 and ANP. For all data panels, n = 8 in each groups. *P < 0.05 vs. Sed group, †P < 0.05 vs. Sed + T group, &P < 0.05 vs. EHP + T group, #P < 0.05 vs. Sh-NC + EHP + T group. Abbreviations: c-Ddx, circ-Ddx60; EHP, exercise hypertrophic preconditioning; BW, body weight (g); HW, heart weight (mg); LW, lung weight (mg); TL, tibial length (mm); Sed, sedentary group; EHP, exercise hypertrophic preconditioning; Sh, short hairpin of c-Ddx; T, transverse aortic constriction. NC, negative control.