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. 2022 Jun 16;46:113–121. doi: 10.1016/j.jare.2022.06.005

Fig. 5.

Fig. 5

Identification of proteins bound by c-Ddx and the influence of c-Ddx on the activity of eEF2 and AMPK in mice. (A), Gene ontology analysis of 6 functional proteins bound by c-Ddx in Sham and TAC groups; (B), Verification of the combination of eEF2 and c-Ddx by RNA binding protein immunoprecipitation; (C), Verification of the combination of eEF2 and c-Ddx by molecular docking analysis. The binding score of RNA to eFF2 protein was −325.38 kcal/mol. Right picture represented the electrostatic surface of eEF2 and c-Ddx. (D), The Effects of c-Ddx kncokdown on EHP-induced phosphorylation of eEF2 and AMPK. *P < 0.05 vs. Sed group, †P < 0.05 vs. Sed + T group, &P < 0.05 vs. EHP + T group, #P < 0.05 vs. Sh-NC + EHP + T group. Abbreviations: c-Ddx, circ-Ddx60; AMPK, adenosine 5′-monophosphate activated protein kinase; eEF2, eukaryotic elongation factor 2; Sed, sedentary group; EHP, exercise hypertrophic preconditioning; NC, negative control; Sh, short hairpin of c-Ddx; TAC, transverse aortic constriction.