Fig. 4.

(A) Hierarchical clustering analysis calculated using spearman correlation between anti-hyperglycemic correlation parameters and related genes in the four pathways of KEGG. The intensity of the colour represents the degree of association. (B) Visualization of the correlation network according to the partial correlation between the anti-hyperglycemic correlation parameters and genes. (C) qPCR verified the result of RNA-seq. (D) The linear relationship between qPCR and RNA-Seq. (E-F) Immunoblot bands of 5 proteins in each group. (G) Effects of different groups on the protein expression levels of liver genes related to glycometabolism. Quantification of band integrated density values by Image J. All the relative protein expression levels were normalized by β-actin band gray values of their corresponding groups. *p < 0.05 and ^**p < 0.01 compared with the Normal group; ^#p < 0.05 and ^##p < 0.01 compared with the Model group.