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. 2023 Mar 21;26(4):106443. doi: 10.1016/j.isci.2023.106443

Figure 4.

Figure 4

NKG2D-CAR-T co-expressing CX3CR1 has higher activation in vitro

(A) Representative flow cytometric analysis of CAR-T proportions (NKG2D+EGFR+) and sustainability after CAR-T viral transduction of primary isolated human T cells (n = 6). pCDCAR is the control vector, while NKG2D-IL-15 overexpresses IL-15 and NKG2D-CX3CR1 CAR-T overexpresses CX3CR1.

(B) CAR-T cytotoxic assay of A549 target cells. One-way ANOVA was used to compare the area under the curve of each group, and p values are shown in the graphs.

(C) IFNγ ELISA of supernatants from primary CAR-T cells co-cultured with target A549 cells at the ratio shown. D) tSNE analysis of expression of CD4, EGFR, PD1, Tim3, and LAG3 in CAR-T cells after co-incubation with target cells.