. 2022 Dec 27;7:170–175. doi: 10.1016/j.ijregi.2022.12.007

Table 1.

Details of the primer sets and expected amplicon size.

Microorganism	Primer name	Primer Sequence 5’- 3’	Amplicon size (bp)	Ta
C. trachomatis	16SrRNA-S	CGAGTCGGCATCTAATACTAT	402	44°C
C. trachomatis	16SrRNA-AS	AAAACGACATTTCTGCCGC	402	44°C
N. gonorrhoeae	16SrRNA-S	TAGCCAAGCCGCGAGGC	694	49.3°C
N. gonorrhoeae	16SrRNA-AS	GGCGCAGACGGTTACTTAAGCAGGA	694	49.3°C
M. genitalium	16SrRNA-S	GTAATACATAGGTCGCAAGCGTTATC	771	51.4°C
M. genitalium	16SrRNA-AS	CACCACCTGTCACTCGGTTAACCTC	771	51.4°C
T. vaginalis	β-tubulin gen-S	ATCGTAAAGAGCTTCGTTATCAATG	89	42°C
T. vaginalis	β-tubulingen-AS	GCATGTTGT GCC GGA CAT AAC CAT	89	42°C

Ta is annealing temperature and bp is the base pair.