Table 1.

Advantages and limitations of post-mortem material, animal models, and mono- and co-culture cells models to study human brain development

	Advantages	Limitations
Post-mortem material	Human origin	Fixed temporal representation
	True clinical representation	Material can be scarce
Animal models	Allows for over-time studies	Non-human representation
	Systematic model	Requires genetic modifications
	Scalable	Carry ethical burdens
Mono- and co-culture models	Human origin	Usually two-dimensional with limited three-dimensional capabilities
	Scalable	No complex culture conditions
	Long-term sampling