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. 2023 Apr 15;14:2167. doi: 10.1038/s41467-023-37431-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Domain architectures of: (i) K13 compared to proteins from humans, which demonstrate high sequence similarity to the BTB and Kelch domains, and (ii) apicomplexan AP-2α and KAE compared to human AP-2α as canonical representative. BLASTP E-values indicate relative conservation between common domains. Conserved domains shown in colour; CC, coiled coil. B Maximum-likelihood phylogenies for K13 and KAE. SH-like aLRT branch supports over 0.9 are indicated by black dots, and complete phylogenies are shown in Fig. S11D. C Far-Western blots with immobilized fragments of TgEps15L (arrow) containing either the native (WxxF) or mutated (AxxF) of the predicted AP-2 ear-binding domain (Coomassie-stained gel, black outline). GST-fused ear domains of AP-2 candidate proteins from Mus musculus (Mm) or T. gondii (Tg) (or GST alone as negative control) were allowed to bind to the Esp15L fragments and visualized by anti-GST staining. The marker size is 15 kDa. Source data are provided as a Source Data file. D Distribution of K13 complex proteins in myzozoan and related lineages.